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Anemia Diagnosis Questionnaire for Primary Health Physicians
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Identification
Name:
Social Security Number:
Sex:
Please, select an option
Man
Non-pregnant Woman
Pregnant Woman
Date of Birth:
Age:
Settings
Lower normal value for MCV:
ℹ
Some labs use 82 fL
Upper normal value for MCV:
ℹ
Some labs use 98 fL
Normal Hematocrit:
ℹ
Typical reference ranges: • Men (18+): ~42-52% • Women (18+): ~37-47% • Pregnant women: ~33-36% (Ranges may vary by lab.)
Unit System:
SI Units
US Units
ℹ
Toggle between International System (mmol/L, μmol/L) and US Conventional (g/dL, μg/dL) units
Complete Blood Count
Blood Hemoglobin Concentration:
ℹ
WHO defines anemia in adults as: <13 g/dL for men, <12 g/dL for non-pregnant women, <11 g/dL for pregnant women.
MCV:
ℹ
Anemias are classified into: • Microcytic (MCV <80 fL) • Normocytic (80–100 fL) • Macrocytic (>100 fL).
Hematocrit:
Maturation Time:
ℹ
Erythrocytes mature in ~7 days from stem cells. This is a simplified correction factor.
Reticulocyte:
Corrected Reticulocyte:
RP Index:
ℹ
RPI adjusts reticulocyte % for the level of anemia & RBC maturation time.
RBC Count:
Mentzer Index:
ℹ
Mentzer index = MCV / RBC count.
Serum Studies
Iron:
TIBC:
Transferrin:
Transferrin Saturation:
ℹ
Transferrin saturation (%) = (Serum Iron / TIBC) × 100
Ferritin:
Soluble transferrin receptor:
ℹ
Often elevated in iron deficiency; helps distinguish from anemia of chronic disease.
TR-F index:
ℹ
TR-F index = sTfR / ln(Ferritin). A higher index often suggests iron deficiency.
B
12
:
Folic Acid:
Lead:
Haptoglobin:
LDH:
Creatinine:
eGFR:
ℹ
Simplified CKD-EPI formula removing race factor, adapted from Levey & Inker, 2009-2021.
Hematology Lab
Peripheral Blood Smear:
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Round macrocytes
Pseudo-Pelger-Huet anomaly
RBC Morphology:
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Spherocytes
Elliptocytes
Stomatocytes
Bite cells (Heinz bodies)
Sickle cells
Polychromasia
Coarse basophilic stippling
>1% fragmented cells
Defect by infection/drugs:
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Yes
No
GPI anchor deficiency (Flow Cytometry):
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Yes
No
ℹ
If hemolysis is not due to infection/drugs, check for GPI-anchor deficiency (PNH).
EMA / Osmotic Fragility:
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Positive
Negative
ℹ
Positive test suggests hereditary spherocytosis.
Coombs test:
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Coombs test is negative
Coombs test positive (IgG+, C3d+) + spherocytes
Coombs test positive (C3d+) + RBC agglutination
Hemoglobin Electrophoresis:
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HbF > 1% and/or HbA2 > 3.5%
HbF <= 1% and HbA2 <= 3.5%
Bone marrow exam/biopsy:
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Infiltration
Hypoplasia/Aplasia
Confirmed MDS
Urine analysis:
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Hemoglobinuria present
Hemoglobinuria not present
Medical History
Alcoholism, liver disease, or thyroid disease
Chronic inflammation, chronic disease, or malignancy
Medicines that may cause macrocytic anemia
Burn injury
Snake or insect bites
Prosthetic valve or arterial graft
Malignant hypertension
Vasculitis
Myelodysplastic syndrome
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